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polymorphprep tm (lot: 00121)  (Axis-Shield Diagnostics)

 
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    Structured Review

    Axis-Shield Diagnostics polymorphprep tm (lot: 00121)
    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
    Polymorphprep Tm (Lot: 00121), supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polymorphprep+tm/polymorphprep/pmc11719704-155-0-7
    Average 90 stars, based on 1 article reviews
    polymorphprep tm (lot: 00121) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways"

    Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26010143

    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
    Figure Legend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Techniques Used: Extraction, Isolation

    Related Articles

    Isolation:

    Article Title: Interferon-γ induces interleukin-6 production by neutrophils via the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway.
    Article Snippet: .. Neutrophils were isolated by density gradient centrifugation using Polymorphprep TM (Axis-Shield, Oslo, Norway). ..

    Article Title: Inhibition of CEACAM1 expression in cytokine-activated neutrophils using JAK inhibitors.
    Article Snippet: .. The blood was layered on a Polymorphprep TM (Axis-Shield, Oslo, Norway) cushion and cells were isolated according to the manufacturer’s protocol. ..

    Article Title: The C-X-C Motif Chemokine Ligand 5, Which Exerts an Antioxidant Role by Inducing HO-1 Expression, Is C-X-C Motif Chemokine Receptor 2-Dependent in Human Prostate Stroma and Cancer Cells
    Article Snippet: .. Neutrophils drawn from subjects’ peripheral blood were isolated by PolymorphPrep TM (Axis-Shield PoC, Oslo, Norway) provided by Dr. I-Hsien Lin, Department of Microbiology and Immunology, Chang Gung University, Taiwan. .. Neutrophils (1.5 × 10 6 ) were suspended in a 200 μL serum-free medium and added to the upper chamber of the transwell, with a pore size of 3.0 μm (PITP01250, Merck Millipore Ltd., Darmstadt, Germany).

    Article Title: Neutrophil extracellular traps and phagocytosis in Pythium insidiosum
    Article Snippet: .. Fresh heparinized blood was layered on Polymorphprep TM (Axis-shield, Norway) and centrifuged at 1,800 rpm for 30 min, 25°C for neutrophil isolation, according to the manufacturer’s instructions. .. The isolated neutrophils were collected, and cells were resuspended in RPMI 1640 medium with 25 mM HEPES and 2 mM L-glutamine (Gibco, USA), supplemented with 10% (v/v) fetal bovine serum (Gibco, USA).

    Gradient Centrifugation:

    Article Title: Interferon-γ induces interleukin-6 production by neutrophils via the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway.
    Article Snippet: .. Neutrophils were isolated by density gradient centrifugation using Polymorphprep TM (Axis-Shield, Oslo, Norway). ..

    Sedimentation:

    Article Title: Cold-Inducible RNA-Binding Protein (CIRP) Potentiates Uric Acid-Induced IL-1β Production
    Article Snippet: .. The blood was layered on a Polymorphprep TM (Axis-Shield, Oslo, Norway) cushion and neutrophils were puri ed using density sedimentation according to the manufacturer’s instructions. ..

    Article Title: Cold-inducible RNA-binding protein (CIRP) potentiates uric acid-induced IL-1β production
    Article Snippet: .. The blood was layered on a Polymorphprep TM (Axis-Shield, Oslo, Norway) cushion and neutrophils were purified using density sedimentation according to the manufacturer’s instructions. ..

    Purification:

    Article Title: Cold-inducible RNA-binding protein (CIRP) potentiates uric acid-induced IL-1β production
    Article Snippet: .. The blood was layered on a Polymorphprep TM (Axis-Shield, Oslo, Norway) cushion and neutrophils were purified using density sedimentation according to the manufacturer’s instructions. ..



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    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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    Image Search Results


    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Journal: International Journal of Molecular Sciences

    Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

    doi: 10.3390/ijms26010143

    Figure Lengend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Article Snippet: PolymorphPrep TM (Lot: 00121) was purchased from Axis-Shield (Serumwerk Bernburg AG, Bernburg, Germany).

    Techniques: Extraction, Isolation