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polymorphprep tm (lot: 00121)  (Axis-Shield Diagnostics)

 
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    Structured Review

    Axis-Shield Diagnostics polymorphprep tm (lot: 00121)
    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
    Polymorphprep Tm (Lot: 00121), supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polymorphprep+tm/pmc11719704-155-0-7?v=Axis-Shield+Diagnostics
    Average 90 stars, based on 1 article reviews
    polymorphprep tm (lot: 00121) - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways"

    Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26010143

    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
    Figure Legend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Techniques Used: Extraction, Isolation



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    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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    Image Search Results


    Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Journal: International Journal of Molecular Sciences

    Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

    doi: 10.3390/ijms26010143

    Figure Lengend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

    Article Snippet: PolymorphPrep TM (Lot: 00121) was purchased from Axis-Shield (Serumwerk Bernburg AG, Bernburg, Germany).

    Techniques: Extraction, Isolation